Single-molecular tracking allows us to observe the motion of a single molecule for an extended period of time inside live cells or in nanostructures. But the current single-molecule tracking microscopes cannot probe the molecular association/dissociation events along the molecular trajectories in 3D space. We plan to address this challenge through innovative microscope design, computer simulation, feedback control, system integration, and materials technologies. Our goal is to create a new 3D molecular tracking method that can not only follow the movement of a single biomolecule inside live cells for tens of minutes but can also observe biomolecule’s weak interactions or transient binding (kd ~ µM) with surrounding molecules during trafficking. Using this enabling technique, we hope to map molecular trafficking highways, molecular association/dissociation hotspots, and cellular compartments or subcellular structures all together in live cells. This chemical measurement and imaging capability has never been realized before and, if successfully implemented, will greatly facilitate our understanding of receptor trafficking pathways and signaling networks at unprecedented spatial and temporal resolutions.
C.-K. Chiou, Y.-L. Liu, P.-J. Huang, P.-H. Tsou, C.-T Chen, H.-H. Lee, Y.-N. Wang, J.L. Hsu, J.-F. Lee, T.E. Yankeelov, J. Kameoka*, H.-C. Yeh*, and M.-C. Hung*, "Digital receptor occupancy assay in quantifying on- and off-target binding affinities of therapeutic antibodies", ACS Sensors 5, 296-302, 2020.
Y.-L. Liu*, E.P. Perillo, P. Ang, M. Kim, D.T. Nguyen, K. Blocher, Y.-A. Chen, C. Liu, A.M. Hassan, H.T. Vu, A.K. Dunn and H.-C. Yeh* ( co-corresponding authors), "Development of three-dimensional two-color dual-particle tracking microscope and its applications in monitoring DNA conformational changes and antibody-conjugated nanoparticle landings on the plasma membrane", ACS Nano, 2020.
M.S. Blevins, D. Kim, C.M. Crittenden, S. Hong, H.-C. Yeh, J.T. Petty and J.S. Brodbelt, "Footprints of nanoscale DNA-silver cluster chromophores via activated-electron photodetachment mass spectrometry", ACS Nano 13, 14070-14079, 2019.
July 15, 2020
Our research is sponsored by the NSF Nanoscale Interactions Program.
April 13, 2020
Nina received the University Graduate Continuing Fellowship for 2020-2021. Such an award speaks highly of Nina's record of accomplishments as a graduate student. Congratulations!
March 2, 2020
Pranav Anbarasu won $500 Undergraduate Research Fellowship. Congratulations!
February 29, 2020
Invited by the Taiwanese Student Association, Dr. Yeh gave a career development talk at Johns Hopkins University.
February 19, 2020
Michael gave a poster presentation on "Massively parallel activator selection of NanoCluster Beacons" at the BPS conference.
Tim Yeh, Ph.D. (Hsin-Chih Yeh 葉信志)
Department of Biomedical Engineering
University of Texas at Austin
107 W. Dean Keeton Street Stop C0800
Austin, TX 78712-1801
Office: BME 5.202C
Phone: (512) 471-7931