Single-molecule detection provides researchers with a unique method to probe kinetics of biomolecules in their native environment, without the need to synchronize the molecular states. However, current single-molecule measurements of DNA hybridization kinetics are mostly performed on a surface or inside an electrokinetic trap, which are not physiologically relevant conditions. Recently we demonstrate a time-resolved, 3D single-molecule tracking (3D-SMT) method that that can follow individual DNA molecules diffusing inside a mammalian cell and observe multiple annealing and melting events on the same molecules. By comparing the hybridization kinetics of the same DNA strand in vitro, we found the association constants can be 13- to 163-fold higher in the molecular crowding cellular environment. In contrast to other confocal-feedback 3D single-particle tracking demonstrations, we tracked single DNA reporter strands inside a live cell and measured their annealing-melting kinetics. Although camera-based techniques combined with point-spread function engineering can achieve 3D tracking in live cells, they do not offer any lifetime monitoring capability that can be used to reveal the molecular binding kinetics...
J. Yang, T. Davis, A.S. Kazerouni, Y.-I. Chen, M.J. Bloom, H.-C. Yeh, T.E. Yankeelov and J. Virostko, "Longitudinal FRET imaging of glucose and lactate dynamics and response to therapy in breat cancer cells", under review.
Y.-A. Kuo, C. Jung, Y.-A. Chen, H.-C. Kuo, O.S. Zhao, T.D. Nguyen, J.R. Rybarski, S. Hong, Y.-I. Chen, D.C. Wylie, J.A. Hawkins, J.T. Petty, I.J. Finkelstein and H.-C. Yeh, "Massively parallel selection of NanoCluster Beacons", under review.
Y.-I. Chen, Y.-J. Chang, S.-C. Liao, T.D. Nguyen, J. Yang, Y.-A. Kuo, S. Hong, H.-C. Li, Y.-L. Liu, H.G. Rylander III, S.R. Santacruz, T.E. Yankeelov, and H.-C. Yeh, "Deep learning improves complex lifetime estimation in fluorescence lifetime imaging microscopy", under review.
September 9, 2021
Soonwoo won the Earnest E. and Elsie M. Clawson Endowed Scholarship in Engineering for 2021-2022. Congratulations!
August 23, 2021
Dr. Yeh served as a poster competition judge at the 4th BME graduate student retreat.
August 23, 2021
Michael won a poster award at the 2021 BME Graduate Student Retreat. Congratulations!
July 7, 2021
Soonwoo received the 2021 BME Professional Development Award for attending the biannual Biosensors conference. Congratulations!
July 1, 2021
Nina received $10k from Texas Health Catalyst Porgram for supporting the development of her lifetime imaging algorithm flimGANE. Congratulations!.
Tim Yeh, Ph.D. (Hsin-Chih Yeh 葉信志)
Department of Biomedical Engineering
University of Texas at Austin
107 W. Dean Keeton Street Stop C0800
Austin, TX 78712-1801
Office: BME 5.202C
Phone: (512) 471-7931