Single-molecular tracking allows us to observe the motion of a single molecule for an extended period of time inside live cells or in nanostructures. But the current single-molecule tracking microscopes cannot probe the molecular association/dissociation events along the molecular trajectories in 3D space. We plan to address this challenge through innovative microscope design, computer simulation, feedback control, system integration, and materials technologies. Our goal is to create a new 3D molecular tracking method that can not only follow the movement of a single biomolecule inside live cells for tens of minutes but can also observe biomolecule’s weak interactions or transient binding (kd ~ µM) with surrounding molecules during trafficking. Using this enabling technique, we hope to map molecular trafficking highways, molecular association/dissociation hotspots, and cellular compartments or subcellular structures all together in live cells. This chemical measurement and imaging capability has never been realized before and, if successfully implemented, will greatly facilitate our understanding of receptor trafficking pathways and signaling networks at unprecedented spatial and temporal resolutions.
Y.-I. Chen, Y.-J. Chang, S.-C. Liao, T.D. Nguyen, J. Yang, Y.-A. Kuo, S. Hong, H.-C. Li, Y.-L. Liu, H.G. Rylander III, S.R. Santacruz, T.E. Yankeelov, and H.-C. Yeh, "Deep learning improves complex lifetime estimation in fluorescence lifetime imaging microscopy", under review.
I.C. Santos, S. Hong, H.-C. Yeh and J.S. Brodbelt, "Electron photodetachment mass spectrometry to study interactions of silver nanoclusters with DNA monomers and dimers", under review.
M. Kim, S. Hong, T.E. Yankeelov, H.-C. Yeh* and Y.-L. Liu* (co-corresponding authors), "Deep learning-based classification of breast cancer cells using transmembrane receptor dynamics", under review.
November 24, 2020
Soonwoo has received 2021 Virtual Travel Award from Biophysical Society Conference. Congratulations!
November 24, 2020
Our proposal has been awarded a 2020-2021 VPR Research & Creative Grant in the amount of $10,000.
November 22, 2020
Dr. Yeh served as a judge at the 2020 UT BMES case competition.
November 21, 2020
Dr. Yeh gave an invited career development talk at the UT Taiwanese Student Association.
October 29, 2020
Jonathan Mathews won $1,000 Undergraduate Research Fellowship. Congratulations!
Tim Yeh, Ph.D. (Hsin-Chih Yeh 葉信志)
Department of Biomedical Engineering
University of Texas at Austin
107 W. Dean Keeton Street Stop C0800
Austin, TX 78712-1801
Office: BME 5.202C
Phone: (512) 471-7931