Single-molecular tracking allows us to observe the motion of a single molecule for an extended period of time inside live cells or in nanostructures. But the current single-molecule tracking microscopes cannot probe the molecular association/dissociation events along the molecular trajectories in 3D space. We plan to address this challenge through innovative microscope design, computer simulation, feedback control, system integration, and materials technologies. Our goal is to create a new 3D molecular tracking method that can not only follow the movement of a single biomolecule inside live cells for tens of minutes but can also observe biomolecule’s weak interactions or transient binding (kd ~ µM) with surrounding molecules during trafficking. Using this enabling technique, we hope to map molecular trafficking highways, molecular association/dissociation hotspots, and cellular compartments or subcellular structures all together in live cells. This chemical measurement and imaging capability has never been realized before and, if successfully implemented, will greatly facilitate our understanding of receptor trafficking pathways and signaling networks at unprecedented spatial and temporal resolutions.
J. Yang, T. Davis, A.S. Kazerouni, Y.-I. Chen, M.J. Bloom, H.-C. Yeh, T.E. Yankeelov and J. Virostko, "Longitudinal FRET imaging of glucose and lactate dynamics and response to therapy in breat cancer cells", under review.
Y.-A. Kuo, C. Jung, Y.-A. Chen, H.-C. Kuo, O.S. Zhao, T.D. Nguyen, J.R. Rybarski, S. Hong, Y.-I. Chen, D.C. Wylie, J.A. Hawkins, J.T. Petty, I.J. Finkelstein and H.-C. Yeh, "Massively parallel selection of NanoCluster Beacons", under review.
Y.-I. Chen, Y.-J. Chang, S.-C. Liao, T.D. Nguyen, J. Yang, Y.-A. Kuo, S. Hong, H.-C. Li, Y.-L. Liu, H.G. Rylander III, S.R. Santacruz, T.E. Yankeelov, and H.-C. Yeh, "Deep learning improves complex lifetime estimation in fluorescence lifetime imaging microscopy", under review.
July 7, 2021
Soonwoo received the 2021 BME Professional Development Award for attending the biannual Biosensors conference. Congratulations!
July 1, 2021
Nina received $10k from Texas Health Catalyst Porgram for supporting the development of her lifetime imaging algorithm flimGANE. Congratulations!.
June 28, 2021
Together with Dr. Grady Rylander, our research is sponsored by the NIH National Eye Institute.
May 20, 2021
Ted received 2021 CSE PhD Continuing Fellowship. Congratulations!
April 26, 2021
Dr. Yeh gave a lecture on "Advanced Imaging Techniques" at Okinawa Institute of Science and Technology.
Tim Yeh, Ph.D. (Hsin-Chih Yeh 葉信志)
Department of Biomedical Engineering
University of Texas at Austin
107 W. Dean Keeton Street Stop C0800
Austin, TX 78712-1801
Office: BME 5.202C
Phone: (512) 471-7931