Single-molecular tracking allows us to observe the motion of a single molecule for an extended period of time inside live cells or in nanostructures. But the current single-molecule tracking microscopes cannot probe the molecular association/dissociation events along the molecular trajectories in 3D space. We plan to address this challenge through innovative microscope design, computer simulation, feedback control, system integration, and materials technologies. Our goal is to create a new 3D molecular tracking method that can not only follow the movement of a single biomolecule inside live cells for tens of minutes but can also observe biomolecule’s weak interactions or transient binding (kd ~ µM) with surrounding molecules during trafficking. Using this enabling technique, we hope to map molecular trafficking highways, molecular association/dissociation hotspots, and cellular compartments or subcellular structures all together in live cells. This chemical measurement and imaging capability has never been realized before and, if successfully implemented, will greatly facilitate our understanding of receptor trafficking pathways and signaling networks at unprecedented spatial and temporal resolutions.
Y.-A. Kuo, C. Jung, Y.-A. Chen, H.-C. Kuo, O.S. Zhao, T.D. Nguyen, J.R. Rybarski, S. Hong, Y.-I. Chen, D.C. Wylie, J.A. Hawkins, J.T. Petty, I.J. Finkelstein and H.-C. Yeh, "Massively parallel selection of NanoCluster Beacons", under review.
Y.-I. Chen, M.P. Sripati, T.D. Nguyen, Y.-J. Chang, Y.-A. Kuo, S. Hong and H.-C. Yeh, "Recent developments in the characterization of nucleic acids hybridization kinetics", under review.
Y.-I. Chen, Y.-J. Chang, S.-C. Liao, T.D. Nguyen, J. Yang, Y.-A. Kuo, S. Hong, H.-C. Li, Y.-L. Liu, H.G. Rylander III, S.R. Santacruz, T.E. Yankeelov, and H.-C. Yeh, "Deep learning improves complex lifetime estimation in fluorescence lifetime imaging microscopy", under review.
April 26, 2021
Dr. Yeh gave a lecture on "Advanced Imaging Techniques" at Okinawa Institute of Science and Technology.
April 23, 2021
Michael has received 2021 BME professional development award. Congratulations!
March 15, 2021
Dr. Yeh gave an invited seminar at Okinawa Institute of Science and Technology.
March 8, 2021
Dr. Yeh gave a lecture on "Biobarcode Techniques" at Okinawa Institute of Science and Technology.
March 1, 2021
Our research is sponsored by the NSF Biosensing Program.
Tim Yeh, Ph.D. (Hsin-Chih Yeh 葉信志)
Department of Biomedical Engineering
University of Texas at Austin
107 W. Dean Keeton Street Stop C0800
Austin, TX 78712-1801
Office: BME 5.202C
Phone: (512) 471-7931